ORIGINAL ARTICLE
Year : 2022 | Volume
: 8 | Issue : 1 | Page : 110--114
Herbal medicine teng-long-bu-zhong-tang inhibits the growth of human RKO colorectal cancer by regulating apoptosis, senescence, and angiogenesis
Meng-Meng Wei1, Shuang-Shuang Wang2, Jia-Lu Zheng3, Lei Chen4, Xiao Peng4, Jin-Fang Chen4, Hong-Mei An5, Bing Hu4
1 Institute of Traditional Chinese Medicine in Oncology; Department of Oncology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai; Out-patient Department, Pingdu Hospital of Traditional Chinese Medicine, Shandong, China
2 Institute of Traditional Chinese Medicine in Oncology; Department of Oncology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai; Student Affairs Department, Shanghai University of Traditional Chinese Medicine, Shanghai, China
3 Department of Oncology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China
4 Institute of Traditional Chinese Medicine in Oncology; Department of Oncology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China
5 Department of Science and Technology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China
Correspondence Address:
Dr. Bing Hu
725 South Wanping Rd., Shanghai 200032
China
Background: Teng-Long-Bu-Zhong-Tang (TLBZT) is a traditional Chinese herbal medicine used to treat colorectal cancer (CRC). In the present study, we observed the anti-cancer effects of TLBZT on human RKO CRC. Materials and Methods: Mice were subcutaneously transplanted with RKO cells, divided into control, 5-Fu-administered, TLBZT-administered, and TLBZT and 5-Fu combination-administered groups, and treated with 5-Fluorouracil (5-Fu) and/or TLBZT. Apoptosis was detected by TdT-mediated dUTP nick-end labeling assay. The activity of caspase-3,-8, and-9 was detected using specific commercial kits. Cell senescence was assessed using senescence β-galactosidase staining. Protein expression was evaluated by immunohistochemistry. Results: TLBZT inhibited RKO CRC tumor growth, enhanced the anti-cancer effects of 5-Fu, induced apoptosis, and activated caspase-3,-8, and-9. TLBZT induced cell senescence accompanied by the downregulation of cyclin E1 and cyclin-dependent kinase 2 expressions. TLBZT also inhibited angiogenesis and the expression of hypoxia-inducible factor 1 subunit alpha and vascular endothelial growth factor-A. Conclusions: TLBZT inhibited RKO CRC tumor growth and enhanced the anti-cancer effects of 5-Fu, and it could be associated with apoptosis and cell senescence induction, and angiogenesis inhibition.
How to cite this article:
Wei MM, Wang SS, Zheng JL, Chen L, Peng X, Chen JF, An HM, Hu B. Herbal medicine teng-long-bu-zhong-tang inhibits the growth of human RKO colorectal cancer by regulating apoptosis, senescence, and angiogenesis.World J Tradit Chin Med 2022;8:110-114
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How to cite this URL:
Wei MM, Wang SS, Zheng JL, Chen L, Peng X, Chen JF, An HM, Hu B. Herbal medicine teng-long-bu-zhong-tang inhibits the growth of human RKO colorectal cancer by regulating apoptosis, senescence, and angiogenesis. World J Tradit Chin Med [serial online] 2022 [cited 2023 Sep 25 ];8:110-114
Available from: https://www.wjtcm.net/article.asp?issn=2311-8571;year=2022;volume=8;issue=1;spage=110;epage=114;aulast=Wei;type=0 |
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