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ORIGINAL ARTICLE
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Rapid differentiation of Aconiti Kusnezoffii Radix from different geographic origins using ultra-performance liquid chromatography coupled with time-of-flight mass spectrometry


1 National Center for Mass Spectrometry in Changchun, Jilin Province Key Laboratory of Chinese Medicine Chemistry and Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Science, Changchun; College of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei, China
2 National Center for Mass Spectrometry in Changchun, Jilin Province Key Laboratory of Chinese Medicine Chemistry and Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Science; Changchun Sunnytech Co., Ltd., Changchun, China
3 National Center for Mass Spectrometry in Changchun, Jilin Province Key Laboratory of Chinese Medicine Chemistry and Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Science, Changchun, China
4 State Key Laboratory for Quality Research of Chinese Medicines, Macau University of Science and Technology, Taipa, Macao

Correspondence Address:
Zhi-Qiang Liu,
National Center for Mass Spectrometry in Changchun, Jilin Provience Key Laboratory of Chinese Medicine Chemistry and Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Science, Changchun 130022
China
Zi-Feng Pi,
National Center for Mass Spectrometry in Changchun, Jilin Provience Key Laboratory of Chinese Medicine Chemistry and Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Science, Changchun 130022; Changchun Sunnytech Co., Ltd., Changchun 130022
China
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/wjtcm.wjtcm_52_20

Objective: There are different geographic origins of Aconiti Kusnezoffii Radixs (AKRs) sold in the market with different quality. This study aims to establish a rapid analysis method to distinguish the different geographic origins of AKRs and to realize the rapid evaluation of their quality. Methods: An ultra-performance liquid chromatography coupled with time-of-flight mass spectrometry (UPLC-Q-TOF MS) method was utilized to acquire the constituents' information of AKRs from different geographic origins. MSE data and Progenesis QI software were employed to identify the chemical constitutes. Principal component analysis (PCA) was applied to comparing MS data to find the chemical markers of AKRs from different geographic origins. Results: Twenty-three components were detected and 17 out of them were identified, including diester-diterpenoid alkaloids, monoester-diterpenoid alkaloids , and amine-diterpenoid alkaloids. Three pairs of isomers were detected and two of them were distinguished by the retention time of standard samples. Thirteen chemical markers were screened out through PCA and orthogonal partial least square discriminant analysis. Through detecting Napelline or isomer of Napelline (m/z 360.2530) and Aconifine (m/z 662.3170), AKRs from inner Mongolia autonomous could be screened. According to the existence of benzoylaconine (m/z 604.3108) and Indaconitine (m/z 630.3159), it could be confirmed that the AKRs are from Xinjiang Uygur autonomous. AKRs that cannot detect compounds above-mentioned could be from Liaoning or Shanxi Province. Conclusions: The chemical profile could be used not only to distinguish the AKRs from different geographic origins but also to identify the true and false of AKRs. This study lays a foundation for the study of efficacy and toxic of AKRs.


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    -  Pi ZF
    -  Zheng Z
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