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Year : 2023  |  Volume : 9  |  Issue : 3  |  Page : 348-354

Chinese herb formulae inhibit the proliferation of human colon cancer SW480 cells by inducing cell apoptosis

1 Department for Internal Medicine of Traditional Chinese Medicine, Eye Hospital China Academy of Chinese Medical Sciences, Beijing, China
2 Department for Biomedical Sciences, Institute for Chemistry and Biochemistry, University of Veterinary Medicine Vienna, Vienna, Austria

Correspondence Address:
Prof. Wolf-Dieter Rausch
University of Veterinary Medicine Vienna, Vienna 1210
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/2311-8571.369650

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Objective: This study aimed to reveal the antitumor effects of Chinese herbal formulae and the underlying mechanisms in treating colorectal cancer, with a focus on developing traditional Chinese medicine (TCM) as a supplement and alternative therapeutic method for cancers. Materials and Methods: Human colon cancer SW480 cells were treated with three Chinese herbal formulae, Bu Zhong Yi Qi Decoction, Fuzi Lizhong Decoction, and Pulsatilla Decoction at different concentrations (50–600 μg/mL) for 24, 36, and 48 h, respectively. Cell viability was determined using the resazurin reduction assay, and cell survival rate was evaluated using a colony formation assay. After treatment with different concentrations (50–600 μg/mL) of these three formulae for 48 h, the effects of the Chinese herbal formulae on cell apoptosis were investigated using Hoechst/propidium iodide (PI) staining. The positive PI-stained cells were investigated using an EnSpire multilabel plate reader and the positive Hoechst-stained cells were observed under a fluorescence microscope for morphological changes. Results: Bu Zhong Yi Qi Decoction, Fuzi Lizhong Decoction, and Pulsatilla Decoction inhibited SW480 cell proliferation in a dose-and time-dependent manner and induced cell apoptosis. Conclusion: Chinese herbal formulae with a special prescription form of TCM with antitumor effects bring a new perspective in line with the principles of TCM in cancer treatment.

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