| ORIGINAL ARTICLE |
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| Year : 2023 | Volume
: 9
| Issue : 3 | Page : 322-329 |
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Jujuboside a improved energy metabolism in senescent H9c2 cells injured by ischemia, hypoxia, and reperfusion through the CD38/Silent mating type information regulation 2 homolog 3 signaling pathway
Yi-Ran Hu1, Hui-Yan Qu2, Jia-Ying Guo1, Tao Yang3, Hua Zhou3
1 Shuguang Hospital, Shanghai University of Traditional Chinese Medicine; Institute of Cardiovascular Disease, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, China
2 Institute of Cardiovascular Disease, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, China
3 Institute of Cardiovascular Disease, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine; Institute of Cardiovascular Disease, Shanghai Institute of Cardiovascular Disease of Integrated Traditional Chinese and Western Medicine, Shanghai, China
Correspondence Address:
Dr. Hua Zhou
No. 528, Zhangheng Road, Pudong New Area, Shanghai
China
 Source of Support: None, Conflict of Interest: None
DOI: 10.4103/2311-8571.372731
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Objective: This study explored the myocardial protection role of Jujuboside A through an ischemia–hypoxia–reperfusion (IHR) model. Materials and Methods: H9c2 cells were induced by D-galactose (D-gal) and IHR to establish an aging and IHR model. There are four groups of experiments: Control, IHR, D-gal + IHR, and D-gal + IHR + Jujuboside A. Cells viability, Adenosine triphosphate (ATP), reactive oxygen species (ROS), nicotinamide adenine dinucleotide (NAD+), nicotinamide adenine dinucleotide hydride (NADH) content, and NAD+/NADH ratio were detected using biochemical methods. Inflammatory cytokines level was detected by enzyme-linked immunosorbent assay. The expression of CD38, Recombinant NLR Family, pyrin domain-containing protein 3 (NLRP3), and silent mating type information regulation 2 homolog 3 (SIRT3) protein was detected by Western blotting. Results: Compared to the IHR group, cell viability, ATP content, NAD + content, NAD+/NADH ratio, and SIRT3 protein expression decreased, ROS level and inflammatory cytokines increased, and CD38 and NLRP3 proteins raised in the D-gal + IHR group. Compared to the D-gal + IHR group, cell viability, ATP content, NAD + content, NAD+/NADH ratio, and expression of SIRT3 protein increased, ROS level and inflammatory cytokines level decreased, and expression of the CD38 and NLRP3 proteins decreased in the D-gal + IHR + Jujuboside A group. Conclusions: Jujuboside A inhibited the expression of CD38, improved energy metabolism disorder, and mitochondrial function, and decreased inflammation in D-gal-induced H9c2 cells.
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