• Users Online: 2488
  • Print this page
  • Email this page
Year : 2023  |  Volume : 9  |  Issue : 1  |  Page : 71-80

Inhibitory interaction and pharmacological analyses of berries phenolics against Listeria monocytogenes virulent protein internalin B

1 School of Agriculture, Indira Gandhi National Open University, New Delhi, India
2 Department of Bioengineering, Integral University, Lucknow, Uttar Pradesh, India
3 Department of Biotechnology, National Institute of Technology, Raipur, Chhattisgarh, India

Correspondence Address:
Dr. Archana Vimal
Department of Bioengineering, Integral University Lucknow-266026, Uttar Pradesh
Dr. Awanish Kumar
Department of Biotechnology, National Institute of Technology, Raipur-492010, Chhattisgarh
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/2311-8571.364413

Rights and Permissions

Background: Traditional plants, their parts, and phytochemicals obtained from them are beneficial for human beings. They are used as potent antimicrobials, but very little research is conducted on the use of traditional medicine against food-borne infection. Different berry plants are rich in phenolic compounds and conventionally known to have many properties such as antioxidants, anti-carcinogenic, anti-inflammatory, anti-bacterial, and anti-diabetics. However, only limited polyphenols are known for their antilisterial effect. The present study aimed to explore the antimicrobial efficacy of phenolic compounds of berries for the treatment of food-borne infection caused by the bacteria Listeria monocytogenes. Materials and Methods: Molecular docking studies employing the SwissDOCK server were performed to evaluate the antimicrobial activity of phenolic compounds obtained from different varieties of berries. Internalin B (InlB), a virulence protein of L. monocytogenes was selected as a target. The absorption, distribution, metabolism, excretion, and toxicity profiling of each test ligand was done through the SwissADME tool. Results: Among all the test ligands, p-coumaric acid, epicatechins, chlorogenic acid, and quercetin showed better binding efficiency with the target protein InlB. The binding energy obtained for quercetin, p-coumaric acid, chlorogenic acid, and epicatechins was-8.93,-8.23,-8.18,-7.58, kcal/mol, respectively. Quercetin and p-coumaric acid were forming 4 H-bonds, whereas chlorogenic acid and epicatechins were forming 3-H bonds inside the binding pocket. Conclusion: In a nutshell, analyses indicated that identified ligands have the potential to block the virulent protein InlB of L. monocytogenes and help combat Listeria infection. These phenolic compounds could be a substitute for synthetic antimicrobials and can be used in food preservation and combat food-borne diseases. However, future in-depth in vitro and in vivo analysis is needed to get more information on these four phenolic ligands of berries.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded103    
    Comments [Add]    

Recommend this journal