ORIGINAL ARTICLE |
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Year : 2022 | Volume
: 8
| Issue : 3 | Page : 446-452 |
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High-performance liquid chromatography fingerprint of marine traditional Chinese medicine haliotidis
Qing Zhao1, Shu-Yue Song1, Meng-Qi Zhang1, Xin Li1, Yang Liu2, Chang-Yun Wang1
1 Key Laboratory of Marine Drugs, The Ministry of Education of China, School of Medicine and Pharmacy, Ocean University of China; Laboratory for Marine Drugs and Bioproducts, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China 2 Insect Biotechnology, Justus Liebig University Giessen, Giessen, Germany
Correspondence Address:
Prof. Chang-Yun Wang Key Laboratory of Marine Drugs, the Ministry of Education of China, School of Medicine and Pharmacy, Ocean University of China; Laboratory for Marine Drugs and Bioproducts, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237 China
 Source of Support: None, Conflict of Interest: None
DOI: 10.4103/wjtcm.wjtcm_71_21
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Objective: Haliotidis, the dried muscle of abalone, has long been used as a marine traditional Chinese medicine (TCM) due to its high medicinal and nutritive values, whereas few reports contributed to its quality control. This study aimed to establish the high-performance liquid chromatography (HPLC) fingerprints of Haliotidis. Materials and Methods: The HPLC fingerprints of Haliotidis, the manufacturing products of dried muscle of abalone, were analyzed by reverse-phase HPLC, using a YMC HPLC C18 column (250 mm × 4.6 mm, 5 μm). The gradient elution mode was applied with mobile phase starting from 5% acetonitrile and 0.1% phosphoric acid aqueous solution to 100% acetonitrile in 70 min at a flow rate of 1.0 mL/min. The detection wavelength was set at 190 nm. Results: Through compared the HPLC fingerprint chromatograms of 14 batches of Haliotidis, 25 peaks were assigned as the characteristic common peaks, of which one index component L-phenylalanine was identified by comparison with its standard substance. The established HPLC fingerprint analysis method of Haliotidis was validated with good precision, repeatability, and stability. Based on the above study, a reference HPLC fingerprint chromatogram of Haliotidis was proposed. Conclusions: It is the first time to establish the HPLC fingerprint chromatogram of TCM Haliotidis, which could contribute to the establishment of its quality control system.
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