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Relaxant effect of bioactive component compatibility of San-ao decoction on In vitro guinea pig airway smooth muscle: A dose-response relationship study
Wen-Jie Song1, Yan-Ling Fu2, Sheng-Lou Ni3, Jia-Jia Fan2, Qian Du4, Hao Zheng1
1 Chinese Medical College, School of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Jing Hai District, Tianjin, China
2 Chinese Medical College, School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Chaoyang District, Beijing, China
3 Periodicals Publishing Center, Beijing University of Chinese Medicine, Chaoyang District, Beijing, China
4 Wenzhou Traditional Chinese Medicine Hospital, Wenzhou, China
Correspondence Address:
Sheng-Lou Ni
Periodicals Publishing Center, Beijing University of Chinese Medicine, 11 Beisanhuan East Road, Chaoyang District, Beijing - 100 029
China
Wen-Jie Song
School of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 10 Poyang Hu Rd., Tuan Bo New Town, Jing Hai District, Tianjin, 301617
China
 Source of Support: None, Conflict of Interest: None  | 3 |
DOI: 10.4103/wjtcm.wjtcm_64_21
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Background: Component compatibility is important to the modernization of traditional Chinese medicine. Studies have shown that San-ao decoction (SAD) can treat respiratory diseases by relaxing airway smooth muscle (ASM) and reducing airway hyper-responsiveness. However, whether its bioactive components and compatibility also present with similar relaxant effects remains unknown. This study aims to explore the potential relaxant property, dose-response relationship, and underlying mechanisms of the bioactive component compatibility in SAD. Methods: Network pharmacology was primarily used to identify the bioactive components of SAD and uncover its underlying mechanisms. ASM tension force measuring technique was utilized to verify the relaxant and dose-response effects on in vitro guinea pig ASM. Results: We postulated pseudoephedrine hydrochloride (PH), amygdalin (AM), and diammonium glycyrrhizate (DG) to be the bioactive components of SAD, which could effectively relax ASM in a dose-dependent manner on both acetylcholine-induced and spontaneous contraction. Both PH and AM could lead to DG dose–response curve shift. The regression equation of these three bioactive components was Y = −2.048 × X1 + 0.411 × X2 + 14.052 × X3 (X1, X2, X3 representing PH, AM, and DG, respectively). The underlying mechanisms of these components might be associated with the regulation of smooth muscle contraction. Conclusions: PH, AM, and DG are the bioactive components of SAD, which can relax ASM in a dose–response manner and exert a synergistic effect. Clinically, compatibility of these three bioactive components may serve as a new complementary and alternative treatment for respiratory diseases.
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