| ORIGINAL ARTICLE |
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| Year : 2022 | Volume
: 8
| Issue : 1 | Page : 141-152 |
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Ultra-performance liquid chromatography-quadrupole/time-of-flight mass spectrometry based bile and urine metabonomics study on the ameliorative effects of Curcuma wenyujin rhizoma on acute blood stasis in rats
Min Hao1, Meng-Ting Zhao2, Huang-Jin Tong3, De Ji4, Lin Li4, Lian-Lin Su4, Wei Gu4, Chun-Qin Mao4, Tu-Lin Lu4
1 Chinese Medicine Processing Engineering Center, School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, China
2 Chinese Medicine Processing Laboratory, School of Pharmacy, Guizhou University of Traditional Chinese Medicine, Guizhou, China
3 Department of Pharmacy, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, China
4 Chinese Medicine Processing Laboratory, College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, China
Correspondence Address:
Prof. Chun-Qin Mao
College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing
China
Prof. Tu-Lin Lu
College of pharmacy, Nanjing University of Chinese medicine, Nanjing 210023
China
 Source of Support: None, Conflict of Interest: None
DOI: 10.4103/wjtcm.wjtcm_55_21
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Background: Curcuma wenyujin rhizome (CWR) is a commonly used Chinese herbal medicine for treating blood stasis in China for 1000 of years. However, the underlying mechanism of CWR remains unclear. Aims and Objectives: The purpose of this study is to clarify the bioactive mechanism of CWR in treating blood stasis. Materials and Methods: In this study, pharmacological indexes, including hemorheology and four blood coagulation indexes were tested. Bile and urine metabolomics were engaged by UPLC-Q/TOF-MS. Multivariate statistical analysis were used to screen out differential endogenous metabolites. Results: The results indicated that CWR significantly ameliorated the hemorheology and coagulation functions of acute blood stasis (ABS) model rats. Moreover, 27 endogenous metabolites between the CWR group and the ABS group were screened, and the levels were all improved to certain degrees by CWR preadministration. Metabonomics results indicated that ABS was mainly related to linoleic acid metabolism, arachidonic acid metabolism, glycerophospholipid metabolism, sphingolipid metabolism, pentose and glucuronate intercereasonversions, steroid hormone biosynthesis, and primary bile acid biosynthesis. Conclusion: In a word, the metabolomics method is consistent with the holistic view of traditional Chinese medicine (TCM) that can be a powerful means to illustrate the biological activity mechanism of CWR in treating blood stasis and to offer research demonstration for further study on the effector mechanism of TCM.
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