Metabolomic study of biochemical changes related to toxicity induced by bupleurotoxin using LC-MS coupled with a pattern recognition approach

Zhong-Xiao Zhang; Juan Su; Xin-Ru Liu; Wei-Xing Dai; Shi-Kai Yan; Wei-Dong Zhang

doi:10.15806/j.issn.2311-8571.2014.0008

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MODERN RESEARCH ON CHINESE MATERIA MEDICA

Year : 2015 | Volume : 1 | Issue : 1 | Page : 29-36

Metabolomic study of biochemical changes related to toxicity induced by bupleurotoxin using LC-MS coupled with a pattern recognition approach

Zhong-Xiao Zhang¹, Juan Su¹, Xin-Ru Liu¹, Wei-Xing Dai¹, Shi-Kai Yan², Wei-Dong Zhang³
¹ School of Pharmacy, Second Military Medical University, Shanghai, 200433, P.R. China
² School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200030, P.R. China
³ School of Pharmacy, Second Military Medical University, Shanghai, 200433; School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200030, P.R. China

Correspondence Address:
Wei-Dong Zhang
School of Pharmacy, Second Military Medical University, No. 325 Guohe Road, Shanghai, 200433
P.R. China
Xin-Ru Liu
School of Pharmacy, Second Military Medical University, No. 325 Guohe Road, Shanghai, 200433; School of Pharmacy, Shanghai Jiaotong Univerisity
P.R. China

Source of Support: None, Conflict of Interest: None

DOI: 10.15806/j.issn.2311-8571.2014.0008

The purpose of this study was to detect changes in urine of mice and to clarify the toxicity induced by bupleurotoxin (BETX) using liquid chromatography/quadrupole time-of-flight mass spectrometry (LC-Q-TOF-MS). A procedure for urine analysis using pattern recognition was proposed to evaluate the toxicity induced by BETX in male BALB/c mice. BETX at 2.5 mg/kg was administrated intraperitoneally (i.p.), and urine samples for the metabolomic study were collected from control and BETX experimental groups. Changes in the concentrations of some urine metabolites were detected exclusively in the experimental group. All results suggested that exposure to BETX might cause a disturbance in fatty acid metabolism and the oxidative stress system. These results may not only clarify the underlying mechanism of diverse intoxication effects of BETX but also provide the guidance in preclinical toxicity screening for new drugs.

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